|
KMID : 0613820170270040442
|
|
Journal of Life Science
2017 Volume.27 No. 4 p.442 ~ p.449
|
|
|
Antioxidant Activity and Protective Effects of Cirsium japonicum against Damaged Mouse Liver Cell (BNL CL.2)
|
|
Kim Seon-Jeong
Kang Seung-Mi
Ko Keon-Hee
Nam Sang-Hae
|
|
|
Abstract
|
|
|
|
We analyzed the content of total phenolic and silymarin compounds of Cirsium japonicum (CJ), and its antioxidant activities and Liver protective effects were compared with those of Silybum marianum (SM). The total phenolic content in the aerial part (97.22±5.51 mg/g) of CJ is higher than that in the underground part (85.32±3.06 mg/g). The total silymarin content of CJ was 55.56% of SM, with the underground part (0.47±0.03 mg/g) having higher content than the aerial part (0.18±0.02 mg/g). The antioxidant activity of CJ was generally slightly lower than that of milk thistle, and the underground part of CJ generally had higher activity compared to the aerial part. When CJ extracts were processed at 1 mg/ml, DPPH activities were 83.76±0.60 and 88.28±0.17%, and FRAP activities were 77.63±0.70 and 82.83±0.39% for extracts from aerial part and underground part, respectively. ABTS activities were 68.60±1.24 and 63.41±0.57% for underground and aerial part respectively when extracts were processed at 0.1 mg/ml. The Liver protective effects of CJ were higher in the extracts from underground part compared to the aerial part, Liver cells were damaged by treating them with t-BHP, H2O2 and Ethanol, and then they were treated with 0.2 mg/ml CJ extracts. The survival rates of the damaged liver cells were 49.58±0.34, 76.87±1.10 and 71.73±0.58% respectively, which were higher than the cells not treated with extracts.
|
|
|
KEYWORD
|
|
|
Antioxidant, Cirsium japonicum, liver protective, silymarin, Silybum marianum
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|